Tag

Part:BBa_K3394002

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-11)


Histidine tag (His-tag)

Histidines are very common tags used in the affinity chromatography purification of recombinant proteins. Due to their small size and neutral charge at physiological pH values, his-tags have little interference with the protein structure.

In our design, we added a 6-histidine tag (His-tag) at the N-terminus of our passenger proteins - either cellulase Endo5a or CBD (cellulose binding domain). The passenger proteins were expressed as a cellulolytic complex on the surface of E. coli through AIDA-1 auto-display system.

NCBI Genbank number for the his-tag sequence linked to the cellulase endo5a is HQ657205.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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